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Journal: Nature Communications
Article Title: Single cell spatial transcriptomics track the evolutionary hierarchy and microenvironment remodeling during breast carcinoma invasion
doi: 10.1038/s41467-026-74954-5
Figure Lengend Snippet: a Spatial distributions of oxidative phosphorylation (OXPH) and EMT signatures for Stereo-seq sample P13, P15, P16-1 and P28. The more the color tends towards yellow, the higher the score of the signature is. b Box plots comparing OXPH(up) and EMT(down) scores between DCIS and IBC cells. The tumor cells in this plots are derived from all 9 Stereo-seq samples. c, SPARC , TOP2A and ARAF spatial expression images of P35 (up) and P13 (down). Yellow and Red indicates higher expression compared to green and blue. d , f T47D breast cancer cells transfected with two specific siRNAs (targeting ARAF and TRAF2 ) or non-sense control siRNA. Proliferation assay shows reduced proliferation in siARAF-T47D and siTRAF2-T47D cell groups. The xCELLigence system was used for examining proliferation of these cells. e , g Transwell invasion assays show reduced invasion in siARAF-T47D and siTRAF2-T47D cell groups compared to the non-sense control siRNA group ( n = 4 biological replicates for each experiment. Data are representative of 3 independent experiments). Bar charts represent the quantified counts of invaded cells. Data are presented as mean ± SEM. Statistical analyses were performed using an unpaired two-sided Student’s t-test. Exact P -values are indicated directly above the bars. The detailed statistical results are as follows: siARAF-1 vs. siNC: P = 0.0003, t = 7.485, df = 6; siARAF-2 vs. siNC: P < 0.0001, t = 16.260, df = 6; siTRAF2-1 vs. siNC: P = 0.0101, t = 3.702, df = 6; siTRAF2-2 vs. siNC: P = 0.0088, t = 3.813, df = 6. h , i Bar plots showing the enriched pathways using IBC up-regulated DEGs in Stereo-seq sample P13( h ) and P35( i ). j Pie chart showing cell types and their proportions around IBC cells in Stereo-seq sample P35 (left) and P13 (right). Cells that are within a 15 μm radius of a IBC cell are included in the statistics. k H&E images of sample P35 (left) and P13 (right), with their selected IBC regions (middle). Source data are provided as a Source Data file.
Article Snippet: The primary antibodies used in this study were as follows: ARAF (Abclonal, Cat# A8687, 1:1000),
Techniques: Phospho-proteomics, Derivative Assay, Expressing, Transfection, Control, Proliferation Assay